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Part:BBa_K2364021

Designed by: Asterios Arampatzis   Group: iGEM17_Greece   (2017-10-26)


pCMV-DsRed-J23100-sfGFP-SV40 polyA

DsRed expressed under the control of the CMV constitutive promoter. A bacterial constitutive promoter expressing an sfGFP is used for aid in the cloning process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1320
    Illegal NheI site found at 1343
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1528
  • 1000
    COMPATIBLE WITH RFC[1000]

Transfection experiment of the construct together with a transfection marker in the Caco-2 cell line. We observe DsRed fluorescence and a limited transfection efficiency, possibly due to low transfection rates of Caco-2 cells.

Functional Parameters

Greekom_secondPart1.png

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Categories
Parameters
None